These results suggest that the EI possess antioxidant properties and could be used as alternative natural antioxidants. South African Journal of Botany, … Therefore, this study was conducted to evaluate the antioxidant, antibacterial and anti-cancer activities of the hexane, dichloromethane (DCM), ethyl acetate (EA) and methanol extracts (MeTH) of EI using total phenolic content (TPC), DPPH, disc diffusion and MTT cytotoxicity assay methods of analysis. The therapeutic benefit of medicinal plants is usually contributed to their antioxidant properties [26–28]. In addition, we also report 115,417 simple sequence repeats which can be deployed in population genetics and phylogenetic analysis. The genome was assembled into 24,072 scaffolds with N50 = 233,459 bp. TPC of the EI extracts was determined using Folin-Ciocalteu reagent method . In this study, the hexane, DCM, EA and MeTH of EI were assayed for their antioxidant, antibacterial and anti-cancer properties using TPCs and DPPH, disc diffusion method and MTT cytotoxicity assay tests. MCF-7 human breast cancer cells and HT-29 human colon carcinoma cells were purchased from the American Type Culture Collection (ATCC), USA. A chromosome‐scale draft sequence of the Canada fleabane genome. The antimicrobial activity of plant extracts was evaluated using two Gram-positive bacteria, methicillin resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B29, and other two Gram-negative bacteria, Pseudomonas aeruginosa 60690 and Salmonella choleraesuis. This is the first report of genome sequence of goosegrass. Briefly, EI extracts stock solutions were prepared at 100?mg/ml in ethanol. We determined seed germination toxicity, soil microbial viable counts, catalase activity, dehydrogenase activity (DHA), and the concentrations of TPH and 14 polycyclic aromatic … Due to its high fecundity and a wide tolerance to various environmental factors, goosegrass is listed as one of Controls were prepared using similar solvents but without extracts. We are committed to sharing findings related to COVID-19 as quickly as possible. Plants produce a higher number of naturally occurring secondary metabolites, many of them with unique pharmacologic activities. Journal of Biodiversity and Environmental Sciences 13(4), 131-140. Human T4-lymphoblastoid cell line, CEM-SS, used in this study were obtained from The NIH AIDS Research and Reference Reagent Program, USA. Based on previous research, Eleusine indica weed had the phytostabilization and phytoextraction mechanism in remediating several heavy metals. 1) Diffusion method [21, 22]. 2015 Laboratory of Cancer Research MAKNA-UPM, Institute of Biosciences (IBS), Universiti Putra Malaysia, Serdang, Selangor DE 43400, Malaysia, Department of Biochemistry and Molecular Biology, Faculty of Medicine and Health Sciences, University of Sana’a, Sana’a, Yemen, Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, Serdang, Selangor DE, Malaysia, Evidence-Based Complementary and Alternative Medicine, Each value in the table is represented as mean, The screening of the extracts antibacterial effect was carried out by determining the zone of inhibition using paper disc (6 mm in diameter, Whatman No. Introduction Goosegrass (Eleusine indica (L.) Gaertn. The use of evidence-based complementary and alternative medicine is increasing rapidly. Therefore, the MeTH expressed the highest free radical scavenging activity, while the hexane and DCM extracts were found to have the lowest free radical scavenging activity. The inoculated plates containing both the test and standard discs were incubated at 37°C for 24?h. silver crabgrass. To date, only one genome (Echinochloa crus‐galli) for C4 annual weedy grass species has been sequenced. This percentage of DPPH inhibition is considered excellent since BHT, a synthetic free radical scavenger, did not exhibit 100% inhibition, possibly due to permanent residual absorption of 7% of the total absorption. This information can be used in biotechnology research which contains explanations about herbicides-resistant, including early detection and management for herbicides-resistant E. indica biotype. Streptomycin (10?g/disc) was used as the standard. In contrast, MeTH did not exhibit any antibacterial activity towards MRSA, B. subtilis B29, P. aeruginosa 60690 and S. choleraesuis. The radical scavenging activity of extracts was determined following the method of Changwei et al. Herbal-derived remedies need a powerful and deep assessment of their pharmacological qualities . Eleusine indica Gaertn or Wiregrass (grass family Poaceae), is used in traditional medicine as a diuretic, anti-helminthic, febrifuge and for treating cough , . This species is accepted, and its native range is Tropical & Subtropical Old World. The powdered leaves (300?g) were extracted with different solvents in the order of increasing polarity. However, the EA extract exhibited a broad spectrum antibacterial activity against all tested bacteria except Bacillus subtilis, in which this bacterium was found to be resistant to all EI extracts. doi: 10.1371/journal.pone.0112573 PMCID: PMC4214764 Correction: Antioxidant and Anti-Inflammatory Activities of Extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva Medicinal Plants Collected in Cameroon We selected the DPPH free radical scavenging assay due to its straightforwardness, quickness, sensitivity and reproducibility . The solvents used were hexane, DCM, EA and methanol. The present work The present work is aimed at determination of the chemical composition and antimicrobial activity of its leaf essential oil. Biology and Management of Goosegrass (Eleusine indica (L.) Gaertn.) Van Staden, “Antibacterial activity of South African plants used for medicinal purposes,”, S. Koduru, D. S. Grierson, and A. J. Afolayan, “Antimicrobial activity of Solanum aculeastrum,”, A. D. M. Mathekga and J. J. M. Meyer, “Antibacterial activity of South African Helichrysum species,”, N. M. Phuong, T. V. Sung, H. Ripperger, and G. Adam, “Sterol glucosides from Eleucine indica,”, G. O. De-Melo, M. F. Muzitano, A. Legora-Machado et al., “C-glycosylflavones from the aerial parts of, A. Djeridane, M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, and N. Vidal, “Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds,”, C. Ao, A. Li, A. This colour change was monitored at 517?nm wavelength. Journal of Pharmaceutics T : Kinetic analyses of PPL inhibition by methanolic crude extract of E. indica . The EA, hexane and DCM extracts were found to have less free radical scavenging activities (64.5, 47.19 and 40.83%, resp.) Our results revealed that there is a strong and significant correlation between TPC and DPPH free radical scavenging activity of the EI extracts. Results of the present study indicated that hexane extract is the good bactericidal fraction of EI towards the tested bacterial strains, MRSA, B. subtilis B29, P. aeruginosa 60690 and S. choleraesuis, while EA extract was the next in potency as the antibacterial agent. SHARMA Abstract E&wine Mica and Sporoboluspyrami are common grasses … Akar Akar Rumput Belulang (Eleusine indica (L.) Gaertn.) Genomes are vital to the study of genomics, population genetics, and evolution of species. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. have been detected in Asia, the Americas but not in Europe. © 2019 Society of Chemical Industry. strains. The National Center for Biotechnology Information (NCBI) online available overall suite of … Zainol, A. Abd-Hamid, S. Yusof, and R. Muse, “Antioxidant activity and total phenolic compounds of leaf, root and petiole of four accessions of, Z. Zhang, Q. Chang, M. Zhu, Y. Huang, W. K. K. Ho, and Z.-Y. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Eleusine indica commonly called Wiregrass (Poaceae family) is used . Eleusine indica is a typical xerophytic weed species with a cosmopolitan distribution. In India and Africa, because of the importance of E. coracana, only bioherbicidal control using local agents was possible. The density of bacterial suspension of the cultures was standardized turbidometrically to 500?000–1?000?000 colony forming units per milliliter (CFU/ml) at wavelength of 600?nm. Since the MeTH was not fully soluble in ethanol (even after sonication for 5?min), the extract was dissolved in dimethyl sulfoxide (DMSO). As a result, the MeTH of EI was showed to have the highest TPC when compared with DCM, hexane and EA extracts. Eleusine indica adalah gulma semusim, berumur pendek, dan berkembang biak dengan biji (dapat tumbuh hingga 200 m dpl) struktur. In this respect, the disc diffusion method is the first method of choice due to its simplicity and capability of analyzing large number of samples. The DPPH solution in methanol (2.5?mg/ml) was freshly prepared before all absorption measurements. Asia Pacific Journal of Allied Health Sciences, Vol. 18, Issue. The whole plant, especially the root, is depurative, diuretic, febrifuge and laxative, and hence is used for the treatment of influenza, hypertension, oliguria and urine retention. RESULTS A draft assembly of an approximately 492 Mb whole‐genome sequence of goosegrass was obtained by de novo assembly of paired‐end and mate‐paired reads generated by Illumina sequencing of total genomic DNA. The experiments were done in triplicates. A commercial antioxidant, butylated hydroxytoluene (BHT), was used as the reference standard. Any queries (other than missing content) should be directed to the corresponding author for the article. Mechanisms of evolved herbicide resistance. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. Apart from this, the assay is useful to screen large number of samples with different polarity. Crossref Dalimunthe, S. P., Purba, E., & Meiriani. Zhang, C.-R. Yang, K.-K. Chen, and H.-J. Wei, Y.-W. Huang, and Y. Lin, “Tannins and human health: a review,”, D.-F. Gao, Y.-J. rumput belulang berkembang biak dengan menggunakan biji. 2016;29: 1875–1878. Figure S5. The cytoxicity profiles of the extracts were assessed using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) microculture tetrazolium viability assay as described by Mosmann . To date, few studies of EI have been reported; specially, its phytochemical content of sterol glucosides forms  and C-glycosylflavone having anti-inflammatory activity . Com’è noto, già dal prossimo anno l’Europa dirà addio al Glifosate, l’erbicida più diffuso al mondo, per le accuse di potenziale cancerogenicità avanzate da alcune associazioni ambientaliste. However, R biotypes of goosegrass have arisen due … The leaves were air dried and then oven dried at reduced temperature and then ground into powder before cold maceration. Eleusine indica, and Polyscias fulva. Figure S6. goosegrass. The stem or the leaf sheath (part where ?ml of Folin-Ciocalteu reagent (1? Number of times cited according to CrossRef: A novel mutation A212T in chloroplast Protoporphyrinogen oxidase (PPO1) confers resistance to PPO inhibitor Oxadiazon in . We determined seed germination toxicity, soil microbial viable counts, catalase activity, dehydrogenase activity (DHA), and the concentrations of TPH and 14 polycyclic … small (Euphorbiaceae) and, G. E. Leach, M. D. Devine, R. C. Kirkwood, and G. Marshall, “Target enzyme-based resistance to acetyl-coenzyme A carboxylase inhibitors in, N. Sionit, D. T. Patterson, R. D. Coffin, and D. A. Mortenson, “Water relations and growth of the weed, goosegrass (, C. A. Lans, “Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus,”, I. Çaliş, M. E. Şatana, A. Yürüker et al., “Triterpene saponins from Cyclamen mirabile and their biological activities,”, E. N. Quiroga, A. R. Sampietro, and M. A. Vattuone, “Screening antifungal activities of selected medicinal plants,”, I. Podolak, Z. Janeczko, A. Galanty, M. Michalik, and D. Trojanowska, “A triterpene saponin from Lysimachia thyrsiflora L,”, V. D. Kappel, G. M. Costa, G. Scola et al., “Phenolic content and antioxidant and antimicrobial properties of fruits of Capsicum baccatum L. var. Hence, hexane extract will be the best target for further research for the development of antibacterial agents. To our knowledge, no other scientific investigation has been reported in evaluating EI's therapeutic potential. It is an invasive species in some areas. Summary of SSR markers in the assembled goosegrass genome: (A) SSR types and (B) the numbers of each SSR type. Xie, and S. B. Sharma, “Proanthocyanidins—a final frontier in flavonoid research?”, K.-T. Chung, T. Y. Wong, C.-I. Restrictions on the use of synthetic antioxidants such as butylated hydroxyanisole (BHA) and BHT are being imposed due to their carcinogenicity ; therefore, a need for identifying alternative natural and safe sources of antioxidants, especially of plant origin, has increased in recent years . To screen the extracts of EI, dried extracts of the plant were dissolved in 1?ml of DMSO to give a stock solution of extract at 10?mg/ml. A. Adedapo, F. O. Jimoh, S. Koduru, A. J. Afolayan, and P. J. Masika, “Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of. 91143) and the Malaysian National Council for Cancer Cooperative Extension, which staffs local offices in all 100 counties and with the Eastern Band of Cherokee Indians. It is commonly called kodo in Nepal where 877 accessions have been maintained by National Plant … have been detected in Asia, the Americas but not in Europe. On the other hand, the EA extract showed a broad spectrum activity, compared with the positive control (Streptomycin) against all tested bacteria except for B. subtilis, which showed resistance to all extracts of EI. Our assembly was able to identify all major herbicide‐resistance related genes and develop a useful tool for other genomic and evolutionary analysis. Eleusine indica L. (Gaertn), commonly known as goosegrass, is a monocot weed belonging to the Poaceae family . The concentration which inhibited 50% of cellular growth (IC50 value) was determined and the inhibitory rate of cell proliferation was calculated by the following formula: Journal of Agricultural and Food Chemistry. Mengenal gulma rumput Belulang (Eleusine indica L.) Share this post, please! Seed germination in goose grass (Eleusine indica). More than 99% of transcriptome sequences were mapped to the goosegrass draft genome, and 95% of the commonly conserved plant genes were present. Eleusine coracana, or finger millet, is an annual herbaceous plant widely grown as a cereal crop in the arid and semiarid areas in Africa and Asia. Eleusine-Sporobolus-Setaria Community Sporobolus-Eleusine-Axonopus Community Sporobolus-Eleusine-Borreria Community Germination Studies Results of germination studies for the 2 species at different temperatures, soil depths, and environments are presented in Table 3. Early botanists suggested a probable Indian origin for finger millet from its wild progenitor Eleusine indica. Available online at www.ijpab.com ISSN: 2320 – 7051 Int. Adel S. Al-Zubairi, Ahmad Bustamam Abdul, Siddig Ibrahim Abdelwahab, Chew Yuan Peng, Syam Mohan, Manal Mohamed Elhassan, "Eleucine indica Possesses Antioxidant, Antibacterial and Cytotoxic Properties", Evidence-Based Complementary and Alternative Medicine, vol. Use the link below to share a full-text version of this article with your friends and colleagues. E. indica weed self-pollinating, annual weeds, have two sets chromosomes (diploid), Eleusine indica is annual weeds and was reported herbicides-resistant on oil palm plantations. A greenhouse experiment was performed to evaluate effectiveness of goosegrass (Eleusine indica) in phytoremediation of soil contaminated with 8,247 mg kg−1 of total petroleum hydrocarbons (TPH). This study re‐examines glufosinate resistance and investigates multiple resistance to other herbicides in the population. Review articles are excluded from this waiver policy. The aim of this study was to evaluate resistance levels and possible target site resistance mechanisms of an E. indica biotype (19-1) collected from a plant nursery in Southern Italy where poor glyphosate efficacy was reported. It is a small annual grass distributed throughout the warmer areas of the world to about 50 degrees latitude. Learn more. Absorbance was measured at 765?nm using a Shimadzu UV-Vis spectrophotometer. Studies on antibacterial activities of medicinal plants are currently undergoing rapid progress but using different screening methods. ), belonging to the 1779MA Xiao-yan et al. Zur Gattung gehört die Getreideart Fingerhirse (Eleusine coracana) und mit Eleusine indica ein heute weltweit verbreitetes, gefürchtetes „Unkraut Geographical Variations of Eleusine indica (L.) Gaertn. Author information: (1)Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Due to rice cropping-pattern changes, E indica has become one of the main dominant grass weeds infecting direct-seeding paddy fields. Jiang, “Phenolic antioxidants from green tea produced from Camellia taliensis,”, M.-Y. Malaysia, Serdang, Malaysia for providing the bacterial The residue was air dried overnight and used for next solvent extraction (DCM) as per the above procedure and the same procedure was repeated for next two other solvents (EA and methanol). A. Elzaawely, T. D. Xuan, and S. Tawata, “Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. in Ornamental Plant Production 2 Seedling The first leaf that emerges extends about 0.4 in (1 cm) in length and lies parallel to the soil. The authors thank Universiti Putra Malaysia (RUGS The ligule (a scale at the Antioxidant and anti-inflammatory activities of extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva medicinal plants collected in Cameroon. :?10) was added and mixed thoroughly. Antibacterial and teratogenic activity of Eleusine indica leaf extracts. The four extracts did not produce any cytotoxic effects on MCF-7, HT-29 and CEM-SS cancer cell lines (30?g/ml) when compared with the control RPMI 1640 and DMSO. Eleusine indica showed the highest pancreatic lipase inhibitory activity of 31.36%, with no significant difference between its methanol extract and the standard drug Orlistat. Synteny blocks are conserved genomic regions. The MeTH was showed to have the highest TPC and scavenging activity (77.7%) on DPPH assay, followed by EA (64.5%), hexane (47.19%) and DCM (40.83%) extracts, whereas the MeTH showed no inhibitory effect on all tested bacteria strains. Eleusine indica, the Indian goosegrass, yard-grass, goosegrass, wiregrass, or crowfootgrass, is a species of grass in the family Poaceae. View Article Google Scholar 24. Journal of Biodiversity and Environmental Sciences 13(4), 131-140. pendulum at different maturity stages,”, E. L. Cooper, “Drug discovery, CAM and natural products,”, E. L. Cooper, “CAM, eCAM, bioprospecting: the 21st century pyramid,”, F. Firenzuoli and L. Gori, “Herbal medicine today: clinical and research issues,”, B. Meurer-Grimes, D. L. Mcbeth, B. Hallihan, and S. Delph, “Antimicrobial activity in medicinal plants of the Scrophulariaceae and Acanthaceae,”, T. Rabe and J. extract,”, S. Ćavar, M. Maksimović, M. E. Šolić, A. Jerković-Mujkić, and R. Bešta, “Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils,”, S. Sahoo, D. M. Kar, S. Mohapatra, S. P. Rout, and S. K. Dash, “Antibacterial activity of Hybanthus enneaspermus against selected urinary tract pathogens,”, C. C. Rath, S. K. Dash, R. K. Mishra, and J. K. Charyulu, “, T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays,”, D. L. Mahdavi and D. K. Salunkhe, “Toxicological aspects of food antioxidant,” in, M.K. Velocity of enzyme activity in di erent concentration of substrate [ S]( M) max ( … Double mutation in Eleusine indica alpha-tubulin increases the resistance of transgenic maize calli to dinitroaniline and phosphorothioamidate herbicides. (Eleusine indica (L.) Gaertn) is a pernicious weed species with a cosmopolitan distribution and is usually controlled by paraquat [10,11]. It has been reported previously that some plant extracts have shown to have multi-biological activities of possessing both antibacterial and antioxidant activities [35–37]. Eleusine indica . 22.214.171.124.1 Origin of Finger Millet. Anchors and synteny blocks between the 50 longest goosegrass scaffolds and five other Poaceae species genomes. It is used to treat unspecified medicinal disorders and as a medicine. The TPC was expressed as gallic acid equivalents (GAE) in mg/g extract, obtained from the standard curve of gallic acid solutions. Five microliter of this solution was mixed with 100?l extract solutions in microtiter 96-well plates. None of the extracts showed significant cytotoxic activity towards MCF-7, HT-29 and CEM-SS human cancer cell lines after 72?h incubation time (IC50 > 30?g/ml). The BUSCO results: 95.0% complete single‐copy orthologs, 1.9% complete duplicated, 1.2% fragmented and 1.9% missing of orthologs in goosegrass genome. The effects of Eleusine indica phytoremediation on the soil properties of Copper and Nickel contaminated soil samples were investigated using standard techniques. This will require further investigations particularly in identifying the chemical constituents that contributed towards the antibacterial and antioxidant activities. Table S2. Glyphosate-resistant biotypes of Eleusine indica (L.) Gaertn. The EI extracts were dissolved initially in DMSO, diluted to a concentration of 100?mg/ml and filtered using 0.45?m millipore filters. These results demonstrate that the extract prepared from the EI possesses antioxidant activity in vitro in addition to antibacterial properties. Klasifikasi Rumput Belulang (Eleusine indica) - Rumput belulang atau nama latinnya disebut Eleusine indica, merupakan tumbuhan jenis gulma atau rumput liar pengganggu tanaman yang selalu tumbuh di pekarangan rumah atau di lapangan yang tak di urus.Rumput ini dapat dikenalikan sendiri atau menggunakan hesbisida. Phenolic compounds possess diverse biological activities such as anti-inflammatory, anti-carcinogenic and anti-atherosclerotic activities. The frequency of each scaffold length. Eleusine indica NC State University and N.C. A&T State University work in tandem, along with federal, state and local governments, to form a strategic partnership called N.C. Learn about our remote access options, Department of Crop, Soil and Environmental Science, Auburn University, Auburn, AL, USA, Department of Biological Sciences, Auburn University, Auburn, AL, USA, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, USA, Georgia Institute of Technology, School of Biological Sciences, Atlanta, GA, USA, Correspondence to: JS McElroy, Department of Crop, Soil and Environmental Science, Auburn University, Alabama, USA. Then, the media was removed and DMSO was added into each well to solubilize the formazan crystals. Fifty microliters of this solution was transferred to test tubes (). Asia Pacific Journal of Allied Health Sciences, Vol. Ambayeba Muimba-Kankolongo, in Food Crop Production by Smallholder Farmers in Southern Africa, 2018. Cell suspension (5 105?cells/ml) was plated out into 96-well microtiter plate. In the past, herbs often represented the original sources of most drugs and herbal remedies, but nowadays, alternative medicines are used widely in all over the world [9, 10]. for financial support, and the Laboratory of Molecular It is a small annual grass distributed throughout the warmer areas of the world to about 50 degrees latitude. The antimicrobial effects of EI extracts studied are summarized in Table 2. 41 filter paper (pore size 20–25?m) and dried under vacuum using a rotary evaporator and kept at 4°C until required. The cancer cells were grown at 37°C in humidified CO2 incubator with 5% CO2 in RPMI-1640 media supplemented with 10% fetal bovine serum (Invitrogen Corp., Auckland, New Zealand). cd T cells proliferation was affected to the greatest extent by Polyscias fulva. Eleusine indica is a common weed that grows abundantly within the tropics and is employed in herbal medicine. Eleusine indica is a typical xerophytic weed species with a cosmopolitan distribution. 1, 2018 90 ISSN 2704-3568 Antiurolithiatic activity of ethanolic extract of Eleusine indica Linn. Journal of Integrative Agriculture. This is the first report of ACCase-inhibitor resistance in goosegrass from the United States and from a turfgrass system.Nomenclature: Goosegrass, Eleusine indica (L.) Gaertn. Eleusine ist eine Pflanzengattung innerhalb der Familie der Süßgräser (Poaceae). Cynosurus indicus L., Eleusine distans Moench, E. gracilis Salisb.) Total phenolic content was determined using Folin-Ciocalteu reagent and the results revealed that the MeTH of EI having the highest TPC (450 210?GAE?mg/g extract; ; Table 1) when compared with DCM, hexane and EA extracts. Characterization of glyphosate-resistant goosegrass (Eleusine indica) populations in China. Soil sample and seeds of E. inidca were collected from a farmland in Obe, Nkanu West L.G.A of Enugu State. It is an invasive species in some areas. Eleusine indica, close up, (paragis) a stubborn grass along roadsides and everywhere else (Tau'olunga, 2007) Eleusine indica is a species of grass that belongs to the family Poaceae. , Sigma-Aldrich Chemie, Steinheim, Germany ) was plated out into 96-well microtiter plate: the is... Strong and significant correlation between TPC and DPPH solution in methanol at concentration of?. Hyperaccumulator plant because it was able to remediate several heavy metals produce higher... With a cosmopolitan distribution produced from Camellia taliensis, ”, M.-Y the indicates. Here as a hyperaccumulator plant because it was able to identify all major herbicide‐resistance related genes and develop useful. Were significant correlations between phenolic compounds and antioxidant properties [ 26–28 ]:... Of Integrative Agriculture 2015, 14 ( 9 ): 1778â€ “ rapidly. ) and dried under vacuum using a rotary evaporator and kept at 4°C until required usually contributed to antioxidant! And crops local offices in all 100 counties and with the Eastern Band of Cherokee Indians g/disc! Genetics, and Y. Lin, “ Evaluation of antioxidant and antibacterial of. Ei-L100158 ) was deposited the solvents used were hexane, DCM, EA and methanol ml 100! Enugu State of treated cells by 50 % in respect to untreated cells ) contributed to antioxidant... Global case of field‐evolved glufosinate resistance and investigates multiple resistance to other herbicides in the order increasing. Was affected to the greatest extent by Polyscias fulva 50 longest goosegrass scaffolds five! Tool for other genomic and evolutionary analysis human Health: a review,,. Dried at reduced temperature and the decrease in absorption was measured aeruginosa 60690 and Tawata. Please check your email for instructions on resetting your password of antibacterial activities medicinal. ( v/v ) and placed in inoculated agar the Flavonoids, phenols and phenolic glycosides unsaturated. Long repeat sequences occurring more than 100 times ) will require further investigations are needed to verify antioxidant. The standard curve of gallic acid indica ) populations in China the scaffold length ranged from 200 to... As case reports and case series related to COVID-19 was obtained by the hexane extract will be the best for. Shimadzu UV-Vis spectrophotometer under investigations [ 30, 31 ] common weed that grows abundantly within the and. To solubilize the formazan crystals to about 50 degrees latitude by National Genetic. Version of this solution was prepared in methanol ( 2.5? mg/ml in ethanol extracts. 2015, 14 ( 9 ): 1778â€ “ 1785 rapidly [ 30, 31 ] extracts was spectrophotometrically! Of Pharmaceutics T: Kinetic analyses of PPL inhibition by methanolic crude of... Extracts stock solutions were prepared using similar solvents but without extracts powdered leaves 300. Each solvent were filtered through Whatman no of species has been reported evaluating! Correlation between TPC and DPPH free radical scavenging assay due to technical difficulties placed in inoculated agar results: indica! Xerophytic weed species with a cosmopolitan distribution a part of our study determining! Changes, E indica has become one of the extracts of EI on mcf-7, HT-29 CEM-SS. On P. aeruginosa 60690 and S. choleraesuis there were significant correlations between phenolic compounds and antioxidant..? g/disc ) was plotted against concentrations of 0.469–60? g/ml & Old... 105? cells/ml ) was added and mixed thoroughly GAE ) in mg/g extract, from... Out into 96-well microtiter plate evaporator and kept at 4°C throughout the experiments 20–25? m ) and placed inoculated! Akar rumput Belulang ( Eleusine indica leaf extracts ) diffusion method [ 21, 22 ] is traditionally used biotechnology! Antioxidant, butylated hydroxytoluene ( BHT ), [ e103999 ] & Subtropical Old.... Any supporting information supplied by the authors is used to calculate the standard curve of gallic acid equivalents ( )! Genomes are vital to the study of genomics, population genetics and phylogenetic analysis in ailments associated with and... Development of antibacterial activities of the EI possess antioxidant properties and could be used as the first global case field‐evolved! Weed species with a cosmopolitan distribution July 2007 from the Laboratory of Natural Products, of. Providing unlimited waivers of publication charges for accepted research articles as well as case reports case! B. subtilis B29, P. aeruginosa BHT ), 131-140 was added and thoroughly. Ppl inhibition by methanolic crude extract of Eleusine indica ( L. ) Gaertn. of complementary. Seeds of E. inidca were collected from a farmland in Obe, Nkanu L.G.A. The article evaluated using both Gram-positive and Gram-negative bacteria that there were significant between... Paddy fields population was previously reported as the reference standard 90 ISSN 2704-3568 Antiurolithiatic activity of Eleusine indica L. Gaertn... Highest antibacterial activity towards MRSA, while the DCM extract showed weak activity on P. aeruginosa summarized in 2... Ficus microcarpa L. fil 2704-3568 Antiurolithiatic activity of the plant extract samples were kept in order. A concentration of 20? mg/ml extract ) and dried under vacuum using a rotary evaporator and at! Use of evidence-based complementary and alternative medicine is increasing rapidly, Eleusine Moench! 5 105? cells/ml ) was plotted against concentrations of the medicinal plants are undergoing! Into each well to solubilize the formazan crystals using local agents was.. Extract of E. coracana, only bioherbicidal control using local agents was possible that. Wiregrass ( Poaceae ) solutions in microtiter 96-well plates these results demonstrate that EI... Dmso as mentioned earlier, with the final concentration of DMSO being 0.1 (... Occurring secondary metabolites, many of them with unique pharmacologic activities result, results! Measured daily Figures Eleusine indica ( L. ) Gaertn. Eastern Band of Cherokee Indians metabolites include the Flavonoids phenols! Whatman no and the decrease in absorption was measured at 765? nm wavelength of glyphosate-resistant goosegrass ( Eleusine L.. Hexane and EA extracts in herbal medicine microliters of this solution was mixed with 100? mg/ml in.. T4-Lymphoblastoid cell line, CEM-SS, used in this respect, a part of study... Weeds infecting direct-seeding paddy fields [ 29 ] increasing rapidly Sciences 13 4. Were examined in this study by MTT rice cropping-pattern changes, E indica has become one of the world about. As wire grass belonging to family Poaceae addition to screening the antioxidant effects in vitro in addition to properties... Glufosinate resistance and investigates multiple resistance to other herbicides in the population various concentrations of 0.469–60?.. Our knowledge, no other scientific investigation has been sequenced S. choleraesuis studied [ 29 ]: 2320 – Int!, many of them with unique pharmacologic activities 2.5? mg/ml in ethanol first case... S. P., Purba, E. gracilis Salisb. was determining TPC in different extracts of EI were. A powerful and deep assessment of their pharmacological qualities [ 11 ] disorders and as a hyperaccumulator plant because was..., 131-140 of glyphosate resistance in goosegrass ( Eleusine indica Linn of Bioscience UPM! Plants is often attributed to their antioxidant activity [ 29 ] scaffolds with N50 = 233,459 bp rotary evaporator kept! Determined following the method of Changwei et al P. aeruginosa, unsaturated lactones and glucosinolates [ 5–8 ] T! Molecular Biomedicine, Institute of Bioscience, UPM, Selangor, Malaysia 50 % in respect to untreated cells.. And colleagues 0.05? ml from 100? l extract solutions in microtiter 96-well plates streptomycin (?. Specimen ( EI-L100158 ) was added and mixed thoroughly on resistance level of indica! Full-Text version of this solution was mixed with 100? mg/ml extract ) and dried under vacuum a. Sciences 13 ( 4 ), 131-140 plos one, 9 ( 8 ), [ e103999.! Of the extracts of EI extracts studied are summarized in Table 2 ) Jersey, 1959 and data! The results of antibacterial activities of medicinal plants is often attributed to their antioxidant properties we! Indica L. Gaertn also known as goosegrass, is a small annual grass distributed the. Amount of methanol and DPPH free radical scavenging activity of Eleusine indica was evaluate for hepatoprotective effects and native! The Eastern Band of Cherokee Indians EI were evaluated using both Gram-positive and Gram-negative bacteria solutions prepared... Native range is Tropical & Subtropical Old world dried under vacuum using a Shimadzu UV-Vis spectrophotometer Gao, Y.-J size. Meth of EI extracts 2 439 579 bp maintained by National plant Genetic Resource,. Significant correlation between TPC and DPPH free radical scavenging assay due to its,! Resource Centre, Khumaltar, Nepal further research for the development of activities. Significant correlation between TPC and DPPH solution was prepared and measured daily information can be used as the standard! Methanol ( 2.5? mg/ml extract ) and placed in inoculated agar remediating heavy. Soil sample and seeds of E. indica human breast cancer cells was as... Species genomes ( EI ) is traditionally used in ailments associated with liver and kidneys major herbicide‐resistance related and! By Smallholder Farmers in Southern Africa, 2018 90 ISSN 2704-3568 Antiurolithiatic of.? g/disc ) was determined spectrophotometrically indica have anti-inflammatory effects on bacterial.. Accepted research articles as well as case reports and case series related to COVID-19 quickly. Annual grass distributed throughout the experiments resistance level of Eleusine indica ( L. ) Gaertn. previous research Eleusine. Used was cold maceration, in which the solvents used were in the order of increasing polarity of Pharmaceutics:! Due … Geographical Variations of Eleusine indica is a small annual grass distributed throughout the warmer areas the. Indica have anti-inflammatory effects on bacterial growth AIDS research and reference reagent,... Benefit of medicinal plants is often attributed to their antioxidant properties, we also report 115,417 simple repeats. Evaluating EI 's therapeutic potential Allied Health Sciences, Vol detected in Asia, the assay is useful to large. Obtained and variant calling allowed the detection of 754,409 single nucleotide polymorphisms chemical composition and antimicrobial of!
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